Establishment of a simple and efficient reverse genetics system for canine adenoviruses using bacterial artificial chromosomes
Hiromichi Matsugo, Tomoya Kobayashi-Kitamura, Haruhiko Kamiki, Hiroho Ishida, Akiko Takenaka-Uema, Shin Murakami, Taisuke Horimoto
Canine adenoviruses (CAdVs) are divided into pathotypes CAdV1 and CAdV2, which cause infectious hepatitis and laryngotracheitis in canid animals, respectively. They can be the backbones of viral vectors that could be applied in recombinant vaccines or for gene transfer in dogs and in serologically naïve humans. Although conventional plasmid-based reverse genetics systems can be used to construct CAdV vectors, their large genome size creates technical difficulties in gene cloning and manipulation. In this study, we established an improved reverse genetics system for CAdVs using bacterial artificial chromosomes (BACs), in which genetic modifications can be efficiently and simply made through BAC recombineering. To validate the utility of this system, we used it to generate CAdV2 with the early region 1 gene deleted. This mutant was robustly generated and attenuated in cell culture. The results suggest that our established BAC-based reverse genetics system for CAdVs would be a useful and powerful tool for basic and advanced practical studies with these viruses.
- : Viruses
- : 10.3390/v12070767
- : http://www.mdpi.com/1999-4915/12/7/767/htm