May 22, 2017

In this study, a strategy for the comprehensive detection and comparison of adducts was applied to find a biomarker for lipid peroxidation-modified proteins in vivo. This adductome approach utilized liquid chromatography with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) methods designed to detect the specific product ions from positively ionized adducts in a selected reaction monitoring mode. Using this procedure, we comprehensively analyzed lysine and histidine adducts generated in the in vitro oxidized low-density lipoproteins (LDL) and observed a prominent increase in several adducts, including a major lysine adduct. Based on the high resolution ESI-MS of the adduct and on the LC-ESI-MS/MS analysis of the synthetic adduct candidates, the major lysine adduct detected in the oxidized LDL was identified as Nϵ-(8-carboxyoctanyl)lysine (COL). Strikingly, a significantly higher amount of COL was detected in the sera from atherosclerosis-prone mice and from patients with hyperlipidemia compared with the controls. ……

For details, please refer to the following link (Japanese)

Paper Information

Takahiro Shibata*1,*2, Kazuma Shimizu*1, Keita Hirano*1, Fumie Nakashima*1, Ryosuke Kikuchi*3, Tadashi Matsushita*4 and Koji Uchida*5

Author Affiliations
*1 Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya,Aichi,Japan
*2 PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama ,Japan
the Departments of *3 Medical Technique and *4 Clinical Laboratory and Blood Transfusion, Nagoya University Hospital, Nagoya ,Aichi,Japan
*5 Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

DOI: 10.1074/jbc.M116.762609.